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. 2018 Apr 22;10(4):213. doi: 10.3390/v10040213

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression profiles of PIWI protein family members of Aedes albopictus. (A) RNA-seq expression profile. tBLASTx was performed against Ae. albopictus and midgut RNA-seq data spanning serial time points post-DENV-2 infection (SRP077936). Counts were normalized to the number of million reads per sample; (B) RT-PCR spatial expression analysis of PIWI protein family member genes. Ae. albopictus Rps7 was used as an internal control. The analyses were performed on the following samples: 0C: uninfected carcass; 0Mg: uninfected midgut; 1MgC: control midgut at 1 dpi; 1MgD: DENV-infected midgut at 1 dpi; 1CC: control carcass at 1 dpi; 1CD: DENV-infected carcass at 1 dpi; 5MgC: control midgut at 5 dpi; 5MgD: DENV-infected midgut at 5 dpi; 5CC: control carcass at 5 dpi; 5CD: DENV-infected carcass at 5 dpi.