Figure 8.

Graphical summary of the correlation between the mean particle sizes of TiO₂ NP and their impact on cytotoxicity in ALI culture of A549 cells. In these graphs the correlation between the biological responses induced by TiO₂ NP, under aerosol condition, and the mean particle size of NM-100 (100 nm) and NM-101 (6.5 nm), is shown. For convenience, we reported the results obtained by aerosol exposure, as we did not find any significant differences between the two exposure methods used. (A) 1 µg/mL of NM-100 and NM-101; (B) 10 µg/mL of NM-100 and NM-101; (C) 25 µg/mL of NM-100 and NM-101. The legend for all the three Panels is showed in Panel A. Abbreviations: IL-1β, interleukin-1beta; IL-6, interleukin-6; TNF-α, tumor necrosis factor alpha.