FIG. 2.

The Raman spectra of the A549 cell cultured inside the microfluidic device (green); and cultured on the MgF₂ substrate in a dish (Red) with 100% laser power; as well as Raman spectrum of the MgF₂ substrate at 100% laser power (Black); and Raman spectrum of the PDMS substrate at 10% laser power (Blue). All spectra collected under 63X aqueous lens with 10 s exposure time and 100% laser power (a). The Raman spectra of A549 cells cultured inside the microfluidic device (red); and cultured on the MgF₂ substrate in the dish (Green), both subtracted with MgF₂ substrate background spectra, several peak intensity differences are highlighted in cyan color (b).