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. 2018 Mar 30;8(4):56. doi: 10.3390/brainsci8040056

Figure 4.

Figure 4

Effects of LDHA siRNA on UW402. UW402 cells were untreated, treated with vehicle control (jet prime transfection reagent), LDHA siRNA and jet prime transfection reagent, or control siRNA and jet prime transfection reagent. (A,B) Western blot of lysates prepared from UW402 cells 72–96 h after transfection. 25µg of protein was loaded into each well and the blot was probed for LDHA (37 kDa) and cyclophillin A (18 kDa) as a loading control. (A) Western blot bands; (B) Semiquantitative analysis of LDHA expression using image J software. LDHA expression was normalised to Cyclophilin expression. LDHA expression is represented as a fold change from control siRNA LDHA expression at each time point; (C) Lactate concentrations of lysates prepared from UW402 cells 72–96 h after transfection. Lactate concentrations were normalised to the protein concentrations of each sample. Graphs show mean with SEM. All samples were compared to each other at each time point, **** p ≤ 0.0001.