Fig. 2.

Cytokines are overproduced in zc3h12a^−/− CD4 T cells in vivo. Frequency of cytokine⁺ and CD44⁺ splenic cells gated on CD4 after stimulation with P/I (A and B) (n=5). (C) Summary of cytokine-expressing cells as shown in (B) (three independent experiments with 3–6 mice per group). (D) Intracellular cytokine expression in total CD4 T cells after stimulation with anti-CD3/CD28 Abs for 4 days, resting for 2 days and then re-stimulation with P/I (6 mice per group). (E) Total CD4⁺ T cells were stimulated by anti-CD3 Ab with or without anti-CD28 Ab for 48 h and supernatants were used to detect cytokines by ELISA (3 mice per group). *: p< 0.05, **: p<0.01, ***: p< 0.001.