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Purification table of α-AI protein from Barnyard millet seed (variety PRJ1)
| Purification steps | Volume (ml) | Total inhibitory activity (U/ml) | Protein (mg/ml) | Specific activity (U/mg) | Fold purification | Yield (%) |
|---|---|---|---|---|---|---|
| Crude extract | 121 | 25.69 | 0.92 | 27.87 | 1 | 100 |
| Ammonium sulfate precipitation | 6.5 | 363.64 | 7.16 | 50.77 | 1.82 | 76 |
| Ion-exchange | 2 | 397.60 | 1.47 | 271.03 | 9.73 | 26 |
| Size exclusion | 5 | 267.90 | 0.43 | 620.14 | 22.25 | 8 |
