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. 2018 Apr 27;8:15. doi: 10.1186/s13395-018-0161-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Selection of candidate miRNAs. a In silico screening of miRNAs that could target NOS1 (TargetScan Human and microRNA.org). Candidate miRNAs are underlined. TLDA (Additional file 2, TLDA A2, B2) (b) and individual RT-qPCR (c) values of candidate miRNA expression in healthy subject biopsies (ctrl, black circle) and BMDd45-55 patients with asymptomatic phenotype (gray circle), moderate phenotype (gray square), severe phenotype (gray triangle), or not determined phenotype (gray hexagon); data are normalized on average of control expression. Lines represent average of each group. Individual RT-qPCR data are expressed as relative quantification using miR-30b as normalizer, normalized on average of control expression