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. 2018 Apr 27;8:15. doi: 10.1186/s13395-018-0161-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — miR-708 and miR-34c overexpression inhibit nNOS expression in transfected control human myoblasts. a miRNA expression in control human myoblasts transfected with non-specific control miRNA (miR-neg, black bar) or indicated selected miRNA (gray bar). Graph represents average of relative quantification of miRNA normalized on SNORD44 expression of 5 (miR-31) or 3 (miR-708 and miR-34c) independent experiments. b Control myoblasts immunolabeled with anti-nNOS (red) antibody, nuclei with Dapi (blue), and imaged by confocal microscopy. Scale bars, 10 μm. Representative of 4 independent experiments. c nNOS immunoblot in transfected control human myoblasts. GAPDH serves as the loading control. Bar graph shows quantification results average of 5 independent experiments. *p ≤ 0.05