Table 2.

Primers used to fragment NOS1-3′UTR

Position in 3′UTR)	Forward primer	Reverse primer
Part 1 (1-1896)	F1-5′TTGTCTAGACTGGACCCTCTTGCCCAGC-3′	R1-5′AAGGATATCCAGGGGAAATTGGGATTAAAGG-3′
Part 2 (1773-3685)	F2-5′-AACTCTAGACTATGACTCACCTTGCTCTGC-3′	R2-5′-ATCGATATCCTTACATGCTCCCTGTCCGTG-3′
Part 3 (3607-5523)	F3-5′-AATTCTAGACTGGTAGCTTCTGGAAGGTAAG-3′	R3-5′AATGATATCGCCACAAGGCAGGGACTGGC-3′
Part 4 (5358-7149)	F4-5′-TAGTCTAGAGAAACACAGGTCTGAGGGTCTG-3′	R4-5′-CCGATATCATTGTAACCATAATGCAAACAAGC-3′

Added restriction sites are indicated in bold characters (XbaI and EcorV)