Skip to main content
. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: J Biomed Mater Res A. 2018 Feb 6;106(6):1476–1487. doi: 10.1002/jbm.a.36350

Figure 2. Effect of pECM on Growth of Cytotrophoblasts.

Figure 2

(a) Effect of pECM on Relative Adhesion. A washing adhesion assay, which is qualitative, was performed to test the relative adhesion of cytotrophoblasts on different substrate coating.34,36,37,57 Our results show that pECM coating significantly increased the adhesion of cytotrophoblasts compared to non-tissue culture treated polystyrene and GelMA coating. In addition, the fraction of adhesion was not significantly different from fibronectin, the most extensively utilized ECM to promote cell adhesion in biomedical research. (b) Effect of pECM on Proliferation of Cytotrophoblasts. The mass of DNA of bioprinted cytotrophoblasts encapsulated in different concentrations of pECM were quantified after 7 days of culture (normalized to day 0). Our results demonstrated a dosage-dependent response of DNA mass and concentration of pECM. Monolayer of cytotrophoblasts grown on GelMA in 2D was used as control to ensure GelMA alone supports cytotrophoblast growth. (c) Representative Live/dead Images of Bioprinted Cytotrophoblasts. The cells are stained with calcein-AM (green) for live cells and propidium iodide (red) for dead cells. The majority of the bioprinted cytotrophoblasts were stained live (green) with very little dead (red). Scale bar = 100 μm. (d) Representative Live/Dead Images of Cytotrophoblasts Demonstrating Aggregate Formation. The cells are stained with calcein-AM (green) for live cells and propidium iodide (red) for dead cells. Scale Bars = 100μm.