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Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis for the recombinant proteins from the isopropy-β-d-thiogalactoside (IPTG)-induced cells of E. coli BL21(DE3)(pJS666). M, protein molecular weight standard; 1, uninduced cells; 2, IPTG-induced cells; 3, Co-purified proteins by Nickel-Nitriloacetic Acid (Ni-NTA) affinity chromatograph under native conditions; 4 and 5, Purified and renatured proteins VapB15 and VapC15-His6, respectively.
