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X. laevis cdh17:eGFP transgenic line is useful for primary culture of kidney tubules. (A) Diagram showing region of embryo that was explanted. (B) Schematic of methodology used to culture cells. Media used for each step is as indicated DFA (Danilchik’s for Amy) and CMFM (Calcium Magnesium Free Media). (C) Image of GFP-positive cells cultured for 24 h in DFA. Cells are outlined using dashed lines.
