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. 2018 Apr 6;9(4):197. doi: 10.3390/genes9040197

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Disruption of kidney development using Daam1 morpholino (MO) can be tracked by cdh17:eGFP. cdh17:eGFP transgenic embryos were injected with Standard or Daam1 morpholino, then stage 42 embryos were assayed for kidney abnormalities. (A) Representative images of kidneys under indicated conditions. (B) Percentage of abnormal kidneys under indicated conditions. (C) Daam1 morpholino was injected into both V2 blastomeres, resulting in the development of edema, which indicates kidney functional defects. Arrow indicates site of edema.