Figure 7.

Histological staining and measurement of the activities of enzymes involved in scavenging and generating reactive oxygen species. (A) Analysis of histologically stained bml and WT rice leaves at the onset of heading, bar = 1 cm (B) Contents of malondialdehyde (MDA), hydrogen peroxide (H₂O₂), and superoxide anions (O₂⁻). (C) Contents of the enzymes superoxide dismutase (SOD), ascorbate peroxidase (POD), and catalase (CAT) in bml and WT at the onset of heading. All data are shown asmeans ± SD of five replicates; * p < 0.05 by Tukey’s method. DAB: diaminobenzidine; NBT: nitro blue tetrazolium.