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. 2018 Apr 12;9(4):209. doi: 10.3390/genes9040209

Figure 3.

Figure 3

Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis of the expression of the transformed gene Gh_A07G0747 (GhTOM). (A) Total RNA isolated from two-week-old cotton plant under normal conditions; (B) Total RNA extracted from salt-stressed cotton seedlings; (C) Polymerase chain reaction (PCR) analysis performed to check 978 bp coding sequence (CDS) integration in transformed T0 generation, number 1–13 transgenic lines, 14 positive control (pWM101-Gh_A07G0747 (GhTOM)), and 15 is the negative control (wild-type, WT). Expression of the transformed genes in transgenic; (D) the transcripts levels of the Gh_A07G0747 (GhTOM) of T2 transgenic lines analysed through qRT-PCR.