Table 1.
Strains and plasmids used in this study.
| STRAIN; PLASMID | Relevant Characteristics | Reference |
|---|---|---|
| Corynebacterium glutamicum STRAINS | ||
| WT | Wild type, ATCC 13032 | [24] |
| ΔcrtEΔidsA | crtE (cg0723) and idsA (cg2384) deletion mutant of ATCC 13032 | [17] |
| ΔcrtOPΔidsAΔcrtB2I’I2 | crtOP (cg0717- cg0723), idsA (cg2384) and crtB2I’I2 (cg2668-cg2672) deletion mutant of ATCC 13032 | this work |
| PAT1 | ΔcrtEΔidsA (pECXT-ispA-PcPS)(pEKEx3) | this work |
| PAT2 | ΔcrtOPΔidsAΔcrtB2I’I2 (pECXT_ispA-PcPS)(pVWEx1) | this work |
| PAT3 | ΔcrtOPΔidsAΔcrtB2I’I2 (pECXT_ispA-PcPS)(pVWEx1_dxs-idi) | this work |
| OTHER STRAINS | ||
| Escherichia coli DH5α | F-thi-1 endA1 hsdr17(r-, m-) supE44 ΔlacU169 (Φ80lacZΔM15) recA1 gyrA96 | [26] |
| PLASMIDS | ||
| pOpt_PcPS | Shuttle vector containing PcPS from Pogostemon cablin codon-optimized for Corynebacterium glutamicum (gene synthesis) (Uniprot Q49SP3) | this work |
| pEC-XT99A (pEC-XT) | TetR, PtrclacIq, pGA1 oriVCg, C. glutamicum/E. coli expression shuttle vector | [27] |
| pEC-XT_ispA-PcPS | pEC-XT derivative for IPTG-inducible expression of ispA from E. coli and codon-optimized PcPS from P. cablin (Uniprot Q49SP3) containing an artificial ribosome binding site | this work |
| pEKEx3 | SpecR, PtaclacIq, pBL1 oriVCg, C. glutamicum/E. coli expression shuttle vector | [28] |
| pEKEx3_araBAD | pEKEx3 derivative for IPTG-inducible expression of the araBAD operon from E. coli containing an artificial ribosome binding site | this work |
| pEKEx3_xylAB | pEKEx3 derivative for IPTG-inducible expression of xylA from Xanthomonas campestris and xylB from C. glutamicum containing an artificial ribosome binding site | [29] |
| pVWEx1 | KmR, PtaclacIq, pHM519 oriVCg, C. glutamicum/E. coli expression shuttle vector | [30] |
| pVWEx1_dxs-idi | pVWEx1 derivative for IPTG-inducible expression of dxs (cg2083) and idi (cg2531) from C. glutamicum containing an artificial ribosome binding site | [18] |
| pK19mobsacB | KmR; E. coli/C. glutamicum shuttle vector for construction of insertion and deletion mutants in C. glutamicum (pK18 oriVEc sacB lacZα) | [31] |
| pK19mobsacBΔcrtE | pK19mobsacB with a crtE (cg0723) deletion construct | [17] |
| pK19mobsacBΔidsA | pK19mobsacB with a idsA (cg2384) deletion construct | [17] |
| pK19mobsacBΔcrtOP | pK19mobsacB with a crtOP (cg0717-cg0723) deletion construct | this work |
| pK19mobsacBΔcrtB2I’I2 | pK19mobsacB with a crtB2I’I2 (cg2668-cg2672) deletion construct | this work |