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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: J Neurochem. 2017 Dec 27;145(3):188–203. doi: 10.1111/jnc.14255

Figure 1. Generation of stable inducible Neurons from human ES cells.

Figure 1

(a) Design of Tol2-recombinase based vectors for conversion of hESCs to neurons. Cells are transfected with the three plasmids along with a transiently expressed to l2 recombinase that allows for gene transfer and stable integration. (b) Schematic showing the time course of generation of siNeurons that includes plating at day -1, dox treatment in E8 media for 2 d and then a media changes to 3N with dox for additional days until analysis. (c) q-RT-PCR analysis showing the time course of Neurogenin 2 expression following Dox treatment. (d) The presence of NEUROG2 protein following dox treatment is shown by Western blot. (e) Representative images of control and siNeurons at day 11 illustrating nuclear immunoreactivity for NEUROG2. The results are a representative of three independent experiments (n=3).