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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: J Neurochem. 2017 Dec 27;145(3):188–203. doi: 10.1111/jnc.14255

Figure 7. FGF2 regulation in siNeurons affect the downstream MAPK signaling pathway.

Figure 7

(a,b) Time-course induction of genes responsive to 2 ng/ml FGF2 over 24h. The IEGs FOS, EGR1 and NXF are induced within 60 min, SPRY4 within 8h and ETV4, NRXN3, GALR1 within 12 h. (c) Immunoblot analyses of FOS and EGR1 protein over a 24 h treatment with 2 ng/ml FGF2. GAPDH is used as a loading control. (d) Inhibition of FOS gene expression upon treatment with PD173074 (red bars), a FGFR1 inhibitor. (e) Inhibition of FOS gene expression upon treatment with PD098059 (red bars), a MAPK inhibitor. (f) Induction of FOS gene expression only in H9-N2 siNeurons (+dox) and not in undifferentiated H9-N2 hESCs (-dox).