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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Mucosal Immunol. 2017 Nov 1;11(2):562–574. doi: 10.1038/mi.2017.74

Figure 3. Comparative analysis of NOX1 variants.

Figure 3

(a, b) Generation of superoxide in colonic epithelial cell line HCT116 reconstituted with multimeric NADPH oxidase complex and expressing wild-type or variant NOX1. ROS was analyzed by L-012-enhanced chemiluminescence in the absence (a) or presence of PMA (b). Chemiluminescence was measured 48 hours post-transfection and recorded over 60 minutes. Results represent eight independent experiments with 3–4 replicates. Each dot represents one replicate. Experiments were normalized to mean relative light units (RLU) without PMA. Line represents mean. Statistical significance was determined with ANOVA and post-test correction performed with Dunnett (multiple comparisons to one single control).