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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Exp Eye Res. 2018 Feb 6;170:101–107. doi: 10.1016/j.exer.2018.02.003

Figure 2. Substratum compliance and latrunculin B (Lat-B) decrease TGFβ1-induced protein expression of αSMA in rabbit corneal fibroblasts (RCFs).

Figure 2

Cells treated with 10 ng/ml TGFβ1 and DMSO expressed significantly greater amounts of α-SMA as substrate stiffness increased. Treatment with Lat-B versus DMSO significantly decreased α-SMA protein expression in RCFs cultured on the intermediate (22 kPa) stiffness in the absence (P = 0.028) or presence (P = 0.018) of TGFβ1. Treatment with Lat-B versus DMSO also significantly decreased (P = 0.048) α-SMA protein expression in RCFs cultured on TCP in the presence of TGFβ1. Mean ± SD of 3 experiments. *P < 0.05 between the different substrates at 0 ng/ml TGFβ1 for DMSO treatment only; a,b,c, P < 0.05 between the different substrates at 10 ng/mLTGFβ1 for DMSO treatment only; † = P < 0.05 for DMSO versus Lat-B.