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Diurnal expression of NMDA receptor subunits in the SCN. Relative expression (ΔΔCT) of NMDA subunits from SCN tissue were assessed at two time points by RT-PCR, (A) midday (ZT 6) and (B) midnight (ZT 18). Midday samples showed age-dependent changes in the expression of NR2A and NR2B (p<0.05, n= 3-7). Midnight samples showed an age-dependent decline in NR2B expression in aged mice relative to the younger cohort (p<0.001).
