Fig. 5. INS1E-hIAPP cells present an impairment in the clearance of damaged mitochondria.

a Histogram representing PINK1 recruitment (fold increase) to mitochondria in whole-cell extracts from INS1E WT, rIAPP, and hIAPP in response to CCCP. b Representative PINK1 immunoblot images from INS1E (WT, rIAPP, and hIAPP) cells treated or not with CCCP (5 µM, 8 h) in mitochondrial-enriched fractions from three independent experiments. TOMM20 was used as a loading control. c Representative immunoblotting images from INS1E (rIAPP and hIAPP) cells treated or not with CCCP in the absence or in the presence of chloroquine. Histograms reflect the fold increase in the ratios of RIESKE/ACTB and LC3B/ACTB protein levels of independent experiments ± s.d. (n = 3), *P < 0.05