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. 2018 Apr 26;6:e4696. doi: 10.7717/peerj.4696

Figure 1. PA, but not OA or LA, is neurotoxic and gliatoxic to SH-SY5Y and T98G cells, and the effects are ameliorated by γ-syn overexpression and exacerbated by PQ treatment.

Figure 1

SH-SY5Y and SH-SY5Y-γ cells were treated with increasing concentrations of PA (A, B), OA (C, D) and LA (E, F) for 24 h (A, C, E) or 48 h (B, D, F). Data represent mean ± S.E.M. of three independent experiments; a and b represent p < 0.05 as compared to the untreated for SH-SY5Y and SH-SY5Y-γ, respectively. (G) Effects of fatty acid treatments in T98G cell line. T98G cells were treated with increasing concentrations (0, 100, 200, 300, 400 and 500 µM) of PA, OA and LA for 48 h; a, b and c represent p < 0.05 as compared to the untreated for PA, OA and LA, respectively. (H) SH-SY5Y cells were co-treated with 300 µM PA and with increasing concentrations (0, 200, 400, 600, and 800 µM) of PQ for 48 h. MTT assay was then performed. Data represent mean ± S.E.M. of three independent experiments; * represents p < 0.05 as compared to 300 µM of PA treatment. (I) SH-SY5Y cells were pre-treated with increasing concentrations (0, 10, 20, 30, 40 and 50 µg/ml) of leptin for 6 h followed by 300 µM PA treatment for 48 h. MTT assay was then performed. Data represent mean ± S.E.M. of three independent experiments; * represents p < 0.05 as compared to untreated.