Figure 7. Impact of SCE on expression levels of ROS- and nitrates-signalling genes.

(A), SsMPK6 (B), SsNLP8 and (C), SsCIPK23. The expression of SsMPK6, SsNLP8 and SsCIPK23 were determined by qRT-PCR on the third and sixth days after treatment. SsACTIN was used as the reference gene. Control, seeds grown in half MS medium. PAs, proanthocyanidins-supplemented half MS medium. SCE, seeds cultivated on half MS medium supplemented with seed-coat extract. Data shown are means ± SD (n = 3). Means with different letters are significantly different at P < 0.05 using Tukey’s HSD post hoc test.