Skip to main content
. Author manuscript; available in PMC: 2019 Feb 21.
Published in final edited form as: ACS Chem Neurosci. 2017 Nov 2;9(2):306–319. doi: 10.1021/acschemneuro.7b00329

Figure 4.

Figure 4

NAB-14 inhibits GluN2C/2D-containing NMDARs in cultured neurons and brain slices. (a) Cultured rat cortical neurons were dissociated and transfected with GFP, GFP and GluN2C, or GFP and GluN2D, and then cultured for 2-5 days. NMDAR current responses were evoked by pressure pulses of NMDA and glycine and recorded by whole-cell voltage-clamp in the absence and presence of NAB-14 (20 μM). (b) The peak amplitude of the current responses were measured and compared between the three transfection groups by one-way ANOVA and post hoc Dunnett’s test [F(2,14) = 16.257, p < 0.001, *GluN2C: p < 0.001, *GluN2D: p = 0.005]. (c,d) NMDAR responses were evoked with pressure pulses of NMDA and glycine at 30 s intervals in acute slices of the rat STN. 20 μM NAB-14 was bath-applied after stable control responses were obtained, then washed out, and 400 μM D,L-APV was applied to ensure the response was NMDAR-mediated. (e) The peak amplitudes of responses in NAB-14 and D,L-APV were plotted as the percent of the control response, and the amplitudes in control and NAB-14-treated conditions were compared by paired t-test (*p < 0.001).