Table A1.
RT qPCR information.
| Gene [Rattus norvegicus (Norway rat)] |
Gene Abbreviation |
Accession Number |
Net Primer Score |
Direction | Primer Sequences |
T (°C) |
Product (bp) |
FASTA (Aligned) |
|---|---|---|---|---|---|---|---|---|
| Actb actin, beta | Bactin | NM_031144.3 | 92 | Forward | TGTGGATTGGT GGCTCTATC |
58 | 149 | AGATGTGGATC GTCCGGCCCCT TTCTAGGCGGA |
| Backward | AGAAAGGGT GT AAAACGCAG |
|||||||
| Gapdh glyceraldehyde-3-phosphate dehydrogenase | Gapdh | NM_017008.4 | 100 | Forward | GGATACTGAGA GCAAGAGAGA |
59 | 106 | ATCCCAACTCG CCCTCACAATT |
| Backward | TTATGGGGTCT GGGATGGAA |
|||||||
| Th tyrosine hydroxylase | Th | NM_012740.3 | 100 | Forward | CTTTGACCCAG ACACAGCA |
59 | 123 | CCAGCCTGTGT ATGACGCCAAC |
| Backward | TGGATACGAGA GGCATAGTTC |
|||||||
| Slc6a3 solute carrier family 6 (neurotransmitter transporter) | Dat | NM_012694.2 | 100 | Forward | CAACTCCACCC TCATCAACC |
60 | 70 | CTCAGGGAAGC ATGCCCTATGT TGCGTGGAGTT TGGCATCAGAG |
| Backward | TTTCTTGCTCC AGGTCTCCC |
|||||||
| Comt catechol-O-methyltransferase | Comt | NM_012531.2 | 100 | Forward | AAGACCGCTAC CTTCCAGAC |
60 | 132 | TGGCCTGCTGC GCTGACAACGT ACTTCCTGGCG |
| Backward | TGCTGCTCCCT CTCACATAC |
|||||||
| Maoa monoamine oxidase A | Mao-a | NM 033653.1 | 100 | Forward | TTTGTGTAGCG TCCAAGGTG |
60 | 103 | GAAGTGTTCCC TCCATGTGATA |
| Backward | AAAGTGCCAAGGGTAGTGTG | |||||||
| Slc6a2 solute carrier family 6 member 2 | Net | NM_031343.1 | 100 | Forward | GCATTGAGAAG GCAGAGAGG |
59 | 135 | GGGACCCCACA ATGCCTGGCCC TGGGGGGTGC |
| Backward | CAGT AGAGCAA GGAAGGCAC |
Gene of interest (abbreviation; http://www.ncbi.nlm.nih.gov/gene), NCB accession number, Net Primer Score (Premier Biosoft), forward and backward primer sequences, melting temperature, product length and FASTA (Aligned Sequence) for each of the genes tested.