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Representative western blot and densitometry analysis of ZO-1 (a), claudin-5 (b) and β-actin, as loading control, proteins levels in bEnd3 cells treated for 24 h with 10 μM of JW74, Phen, SB2, BrG and Tph. Data are presented as mean ± SD of 3 independent experiments. ***P<0.001 compared to control group, ns=not significant.
