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. 2018 Apr 30;9(5):486. doi: 10.1038/s41419-018-0517-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Western blot analysis of E-cadherin, N-cadherin, vimentin, Slug, and Snail in HCC cells with modulation of Rnd1 treated with RhoA siRNA, ectopic expression of RhoA, or stimulated with S1P. Densitometry of western blot in panel. Data are mean ± S.D. (n = 3) and are representative of three independent experiments. Significance was determined using two-way ANOVA. ***P < 0.001. b Representative IF images of E-cadherin and vimentin in HCC cells with modulation of Rnd1 treated with RhoA siRNA, ectopic expression of RhoA, or stimulated with S1P. Scale bar: 50 μm. c The cytoskeleton of HCC cells with modulation of Rnd1 treated with RhoA siRNA, ectopic expression of RhoA, or stimulated with S1P. Scale bar: 20 μm. d Migration and invasion were assessed using transwell assays with or without Matrigel® in HCC cells with modulation of Rnd1 treated with RhoA siRNA, ectopic expression of RhoA, or stimulated with S1P. Scale bar: 100 μm. Panel: quantification of migration and invasion of cells. Data are mean ± S.D. (n = 3) and are representative of three independent experiments. Significance was determined using one-way ANOVA. ***P < 0.001