Figure 2.

Activation of dendritic cells is independent of type I interferon (IFN I) signaling after vaccination with functionalized calcium phosphate (CaP) nanoparticles (NPs). C57BL/6 mice or IFNAR^−/− mice were vaccinated subcutaneously either with phosphate- buffered saline (PBS) or CpG and FV peptide functionalized CaP NPs. 6 h postvaccination, mice were sacrificed and CD11c^high cells from the popliteal lymph node were stained for CD40 and expression of costimulatory molecules CD80 and CD86. The results of two independent experiments are depicted, n = 6. Data represent mean ± SEM.