Figure 7. Current inactivation of Na_V1.7 and Na_V1.3 in cell‐attached and inside‐out configuration.

A, Na_V1.7 currents when the α‐subunit is co‐expressed with β₁ and β₂ in Ins1 cells in response to a step depolarization from −150 to 0 mV in cell‐attached (CA), inside‐out (IO) and 2 and 5 min after excision. B, same as in A, but with Na_V1.3 currents in Ins1 cells. C, voltage dependence of inactivation of Na_V1.3 currents shown in B (same colour code). D, summarized voltage dependence of inactivation of Na_V1.7 currents in Ins1 (black) and CHO cells (red) of indicated number of patches/cells (n). E, same as in D but for Na_V1.3. F, cumulative distribution of Na_V1.3 (red) and Na_V1.7 (black) V _h recorded in cell‐attached patches on Ins1 cells. Data have been normalized (n/∑n) to the total number of patches (n = 18 patches for Na_V1.3 and n = 23 patches for Na_V1.7). For display and to facilitate comparison with inactivation curves, data are shown as (1 − [n/∑n]). The cumulative distributions have been fitted to a double Boltzmann function for Na_V1.3 and a single Boltzmann function for Na_V1.7. [Color figure can be viewed at http://wileyonlinelibrary.com]