Table 3.
NaV1.7–NaV1.3 chimeras expressed in Ins1 and HEK cells
| Monophasic | Biphasic | ||||||
|---|---|---|---|---|---|---|---|
| Cell type | NaV current of NaV1.7–1.3 chimeras | V h (mV) | k | V h1 (mV) | k 1 | V h2 (mV) | k 2 |
| Ins1 | NaV1.7 Control | −90 ± 2 (28) | 9 ± 1 (28) | −94 ± 4 (9) | 9 ± 1 (9) | −63 ± 5 (9) | 8 ± 1 (9) |
| Ins1 | Nterminal domain | −95 ± 1 (7) | 7 ± 1 (7) | −98 ± 1 (2) | 6 ± 1 (2) | −67 ± 1 (2) | 12 ± 2 (2) |
| Ins1 | L1L2 | −88 ± 1 (12) | 9 ± 1 (12) | −86 ± 1 (3) | 7 ± 1 (3) | −49 ± 5 (3) | 13 ± 7 (3) |
| Ins1 | L3 | −96 ± 3 (12) | 8 ± 1 (12) | −98 ± 3 (4) | 9 ± 2 (4) | −50 ± 5 (4) | 9 ± 2 (4) |
| Ins1 | C‐terminal domain | −86 ± 1 (5) | 9 ± 1 (5) | −84 ± 1 (2) | 10 ± 1 (2) | −50 ± 6 (2) | 10 ± 3 (2) |
| HEK | NaV1.7 Control | −60 ± 1 (6) | 8 ± 1 (6) | n/a | n/a | n/a | n/a |
| HEK | N‐terminal domain | −61 ± 4 (6) | 8 ± 1 (6) | n/a | n/a | n/a | n/a |
| HEK | L1L2 | −66 ± 2 (9) | 9 ± 1 (9) | n/a | n/a | n/a | n/a |
| HEK | L3 | −67 ± 3 (11) | 12 ± 1 (11) | n/a | n/a | n/a | n/a |
| HEK | C‐terminal domain | −60 ± 2 (8) | 9 ± 1 (8) | n/a | n/a | n/a | n/a |
V h and k value for the indicated NaV currents. Values represent means ± SEM of indicated number of cells (n). Inactivation curves are presented in Fig. 9. V h represents membrane potential at which inactivation is half‐maximal in cells with monophasic inactivation and k represents the slope factor. In cells with biphasic inactivation, V h1 and V h2 represent the membrane potential at which inactivation is half‐maximal for the current components inactivating at negative and positive membrane potentials, respectively. k 1 and k 2 represent the respective slope factors for the currents inactivating with V h1 and V h2. See Fig. 1 for generation of constructs.