Figure 2. Ano1⁺ ICC‐IM are closely apposed to vAChT⁺ nerve fibres in the gastric fundus.

A–C, varicose VAChT⁺ nerve fibres (A, arrows, green) and Ano1⁺ ICC‐IM (B, arrows, red) within the circular layer (cm) of adult (+/+) fundus muscles. C, merged image showing the close apposition between vAChT⁺ nerve fibres and Ano1⁺ ICC‐IM (arrows). D–F, higher power images of the relationship between vAChT⁺ nerves and Ano1⁺ ICC‐IM. vAChT⁺ nerves were closely apposed to Ano1⁺ ICC‐IM for >250 μm. G–I, gastric fundus muscles of P5 Ano1^+/+ animals also display a similar close morphological relationship between vAChT⁺ nerves and Ano1⁺ ICC‐IM as seen in adult animals. G, vAChT⁺ nerve fibres (arrows, green) and Ano1⁺ ICC‐IM (H, arrows, red) in the circular layer (cm). I, similar to adult tissues, vAChT⁺ nerves were closely apposed to Ano1⁺ ICC‐IM for >250 μm. J–L, at higher magnification, the close apposition between vAChT⁺ nerve fibres and Ano1⁺ ICC‐IM can be readily observed. Scale bars for each series of confocal reconstructions are shown in (C), (F), (I) and (L).