Fig. 4.

Multiplexed sorting of major immune cell types from murine splenocytes by DGS retains cell function. (A) Initial B220⁺, CD8⁺, and CD4⁺ cell frequencies in splenocytes harvested from C57BL/6J mice. (B) Splenocytes were magnetically labeled with DNA-gated antibodies targeting CD19, CD8, and CD4. Individual populations were sequentially recovered by addition of appropriate RP strands (Top, B220⁺ cells; Middle, CD8⁺ cells; Bottom, CD4⁺ cells). (C) Released CD8⁺ cells retain proliferative potential compared with MACS-sorted CD8⁺ cells (Student’s t test, n = 3). Data shown as mean ± SD. (D and E) CD8⁺ cells purified by DGS from OT1 mice produce elevated Granzyme B when coincubated with EG7-OVA target cells compared with EL4 control cells.