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. 2018 Apr 9;115(17):4507–4512. doi: 10.1073/pnas.1716649115

Fig. 2.

Fig. 2.

Effects of JP1 or JP2 knockdown on LTCC currents, gating charges, and Ca2+ transients in C2C12 myotubes. (A) Representative traces of LTCC currents at different membrane potentials. (B) Peak current density–voltage relationships of LTCCs. Mean ± SEM (n = 6–7). *P < 0.05 vs. control. (C) CaV1.1 expression. (D) Representative traces of LTCC gating currents at different membrane potentials. (E) The gating charge density–voltage relationships of LTCCs in C2C12 myotubes. Mean ± SEM (n = 6–7). (F) Absolute number of C2C12 myotubes in chambers responding to field stimulation with Ca2+ transients. Mean ± SEM. Myotubes in seven to nine dishes (153 mm2) were counted. **P < 0.01. (G) Representative traces of Ca2+ transients induced by electrical twitch stimulation. (H) Peak amplitude of twitch Ca2+ transients. Mean ± SEM (n = 12–23). **P < 0.01. (I) Representative traces of Ca2+ transients induced by CPA treatment. (J) Peak amplitude of Ca2+ transients induced by CPA. Mean ± SEM (n = 12–16).