Fig. 3.
Proximal C terminus of CaV1.1 interacts with JPs. (A) Coimmunoprecipitation of CaV1.1, RyR, JP1, and JP2 from solubilized proteins from mouse skeletal muscle microsomes. (B) Pull-down assay with the GST-fused proteins. The N terminus (NT), I–II loop (I-II), II–III loop (II–III), III–IV loop, proximal C terminus (PCT), and distal C terminus (DCT) of CaV1.1 were purified as GST-fused proteins. Proteins were pulled down with the recombinant proteins from mouse skeletal muscle microsomes. (C) Pull-down assay with the GST-fused proteins. A scheme of constructed recombinant proteins of the CaV1.1 C terminus is shown. Bars indicate the regions purified as recombinant proteins. The number on the right side of each bar corresponds to the lane number of the gel image. EF, EF-hand; IQ, IQ-motif; IVS6, the sixth transmembrane segment in domain IV.