Fig. 4.

Validation of RNA-binding activity of several CARIC RBPs. (A) Western blot depicting enrichment of five representative CARIC RBPs. RNase A treatment after click labeling demonstrated the RNA dependence of CARIC capture. (B) Validation of the RNA-binding activity of representative FLAG-tagged CARIC RBPs (VDAC1, NME2, and PSMA7) by CLIP, followed by radiolabeling with T4 polynucleotide kinase and phosphorimaging. Two known RBPs, hnRNPC and MBNL1, were used as positive controls. Anti-FLAG and anti-GFP blots demonstrate equal loading.