Fig. 4.

Effect of glycerol on noscapine production. (A) Schematic illustrating the glycerol utilization mechanism. The pathway facilitating NADPH regeneration is highlighted in blue, and the pathway converting glycerol to dihydroxyacetone phosphate is highlighted in gray. (B) Noscapine titers from CSY1153 under different glycerol concentrations. Ten percent glycerol and trehalose at the indicated ratios were supplemented into YP with 2% dextrose. The strain was cultured at 25 °C for 72 h in the indicated media composition with 10 mM ascorbic acid. Error bars represent SD of three biological replicates.