Fig. 3.

iVα45 T cells are required for host resistance to Mm but not FV3 infection. F₀ transgenic iVα45-Jα1.14 CDR3 KD and control tadpoles (stage 52/53) were sham infected (controls) or either i.p. infected with 1 × 10⁴ pfu FV3 (A and C) or 300,000 cfu Mm (B and D), and survival was monitored daily over a 30- and 60-d period, respectively. Each experiment was repeated three times independently (n = 15 in each cohort) and the results were pooled. Survival was determined using Kaplan–Meier, *P < 0.05, ***P < 0.0005. Shown are dejellied controls (black squares), iVα45-Jα1.14 CDR3shRNA Tg (inverted gray triangles), and Jα1.14sgRNA/CRISPR Tg (gray diamonds).