Fig. 6.
Disruption of other components of the importin-mediated nuclear transportation pathway leads to similar transcription changes. (A) Four independent siRNA pairs targeting the KPNB1 activate reporter gene in B2-1 cells. Cell images (Left), knockdown efficiency (Upper Right), and RGFP values (Lower Right) of each siRNA pair are shown. Error bars represent the SD. (B) Knockdown of KPNAs activates silenced EGFP in B2-1 cells. The percentage of EGFP-expressing cells was measured with FACS. Error bars represent the SD of three biological replicates. (C) Venn diagrams showing the overlap of up-regulated genes (Upper) or down-regulated genes (Lower) in KPNB1- and CSE1L-knockdown cells. (D) Box plot showing the expression level of KPNB1-regulated genes in B2-1 cells treated with siNonTarget, siCSE1L, or siKPNB1.