Turnover of synthetic peptidoglycans by Slt. (A) The six synthetic peptidoglycans and the turnover chemistry by Slt. The reactive glycosidic sites are colored in red, blue, and green. (B–D) LC/MS traces of the reactions of three peptide-stem–containing substrates (1p, 2p, and 3p) with Slt. 1p, 3p, and their turnover products containing pentapeptide. 2p and IIpa* contains tetrapeptide instead of pentapeptide. (E) Time-course of reaction profile (area under the curve vs. time) of Slt with substrate 3p.