Fig. 5. Histone deacetylase 7 (HDAC7) knockdown suppresses transforming growth factor-β1 (TGF-β1)-induced Smad2/3 nuclear translocation in fibroblasts derived from human Peyronie's disease plaque. Representative fluorescent immunocytochemistry of primary human fibroblasts with antibody against total Smad2/3. Fibroblasts were transfected with scramble small interfering RNA (siRNA) or siRNA specific to HDAC7 by using Lipofectamine (GIBCO) reagent for 48 hours and were then treated with TGF-β1 (10 ng/mL) for 1 hour. The cells were washed three times with phosphate-buffered saline and then fixed in 4% paraformaldehyde for 10 minutes at 4℃ and in 100% methanol for 10 minutes at 4℃. Nuclei were labeled with the DNA dye 4,6-diamidino-2-phenylindole (DAPI). Bar indicates 100 µm. Results were similar from four independent experiments.
