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. 2018 Apr 23;9:857. doi: 10.3389/fimmu.2018.00857

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Copyright © 2018 Sherman, Gitschier and Rossi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NK-92MI cell infiltration into A549/GFP tumor spheroids. (A) Representative histological sections of CD45- (red) and e-cadherin-stained (brown) of A549/GFP spheroids (left) and spheroids exposed to NK-92MI cells for 4 h (right) at 200× magnification. (B) Maximum projection confocal images of A549/GFP spheroids (green) subjected to NK-92MI cell (blue) infiltration. Images taken at 20× with a Z stack height of 125 µm via Thermo Scientific™ CellInsight™ CX7. Scale bars are 100 µm (A) and 200 µm (B).