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. 2018 Apr 23;9:857. doi: 10.3389/fimmu.2018.00857

Figure 5.

Figure 5

NK-92MI cell migration and subsequent cytotoxicity toward A549/GFP spheroids. NK-92MI (NK) cell migration (blue) was evaluated in the presence and absence of stromal-cell derived factor-1 (SDF-1α) and/or prostaglandin E2 (PGE) in the medium. Percent migration (left axis) was calculated as a ratio of cells in basolateral chamber compared with total cells seeded in insert. Cytotoxicity of A549/GFP cells (green) was evaluated after exposure to NK-92MI (NK) cells in the presence and absence of SDF-1α and/or PGE in the medium. Percent viability (right axis) was calculated via flow cytometry by enumerating GFP-positive A549 cells relative to spheroids without effector cells. Data are shown as the average of two independent studies, n = 24 wells. *p < 0.001 and **p < 0.0001 in one-way ANOVA with Bonferroni post hoc test.