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. 2018 Apr 23;9:756. doi: 10.3389/fimmu.2018.00756

Figure 1.

Figure 1

Vδ2+ γδT cells express CD161 and produce IFN-γ in response to cytokine stimulation. (A) Frequency of Vδ1+ γδT cells, Vδ2+ γδT cells, and mucosal-associated invariant T (MAIT) (Vα7.2+CD161++) cells in human blood. (B) Representative FACS plot and group averages for expression of CD161. (C) Mean fluorescence intensity (MFI) of CD161 expression on each subset. (D–F) Human peripheral blood mononuclear cells were stimulated for 20 h with the indicated cytokines (50 ng/ml). Brefeldin A and Monensin were added for the final 4 h. (D) Representative FACS plots of IFN-γ production. (E) Production of IFN-γ by each cell subset in response to the indicated cytokines. (F) Production of IFN-γ segregated by CD161 expression for each cell subset. Mean ± SEM. Symbols represent individual donors (N = 9–15), pooled from two individual experiments, or representative of two experiments (C). ***P < 0.001; **P < 0.01; *P < 0.05. Repeated measures paired ANOVA with correction for multiple comparisons (A,B,E) and paired T test (C,F).