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The effect of the different initial medium replacement intervals on cellular morphology and surface markers of BM-MSCs cultured in 5% oxygen condition. (a) Phase contrast images (×100 with scale bar = 200 μm) of BM-MSCs at P0, P1, and P2. (b) The expression of CD29, CD44, Sca-1, CD11b, CD45, and CD19 surface markers of BM-MSCs at P2 determined by flow cytometry.
