Figure 4.

The effect of different initial medium replacement intervals on proliferation capacity and differentiation potential of BM-MSCs cultured in 5% oxygen condition. (a) Representative image of CFU assay (from left to right, the view from one well of the six-well plate, ×40 with scale bar = 500 μm). (b) Quantification of clonogenicity of BM-MSCs by CFU assay. Colonies with an area above 1 mm² were counted. (c) Cell viability determined by CCK8 assay. (d) Oil Red O staining after 2 weeks of adipogenic induction (from left to right, ×100 with scale bar = 200 μm and ×400 with scale bar = 50 μm). (e) The number of adipocytes within a microscopic field (×100) in 1-day or 4-day medium replacement interval group. (f) Quantification of Oil Red O staining. (g) Images of Alizarin Red S staining (from left to right, the view from one 35 mm dish, ×100 with scale bar = 200 μm). (h) Quantification of ALP expression. Results are expressed as mean ± SD from three independent measurements. ^∗p < 0.05 versus 1-day group.