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. 2018 Apr 11;2018:6704583. doi: 10.1155/2018/6704583

Figure 6.

Figure 6

Adipogenic differentiation and osteogenetic differentiation at the earlier passage and the later passage of BM-MSCs cultured in 5% oxygen condition. (a) Representative images for Oil Red O staining at P2 and P12 (from upper to lower, ×100 with scale bar = 200 μm, ×400 with scale bar = 50 μm). (b) The expression of adipogenic differentiation-related genes of BM-MSCs at P12 in BM-MSCs cultured in BM-MSC medium (Con.) and adipogenic induction medium (Diff.). (c) Representative images for Alizarin Red S staining at P2 and P12 (×100 with scale bar = 200 μm, from upper to lower: cultured in BM-MSC medium, and cultured in osteogenetic induction medium). (d) The expression of osteogenetic differentiation-related genes at P12 in BM-MSCs cultured in BM-MSC medium (Con.) and osteogenetic induction medium (Diff.). Results are expressed as mean ± SD from three independent measurements. ∗p < 0.05 versus Con. group.