Table 2.

Summary of studies describing isolate methods and in vitro culture conditions for mouse BM-MSCs.

Isolate method	Operation	Oxygen concentration	Initial medium replacement interval	Reference
Differential adhesion of the bone marrow	BM was flushed out from the bone cavity of femurs and tibias	Not mentioned	72 h	[25, 56]
	BM was flushed out from the bone cavity of femurs and tibias	Not mentioned	48 h	[8, 31, 57]
	BM was flushed out from the bone cavity of femurs and tibias	Not mentioned	24 h	[7, 30]
	BM was flushed out from the bone cavity of femurs and tibias	Not mentioned	3 h	[32]
	Frequent medium change and treatment of the primary cultures with trypsin were used to purify the BM-MSCs	Not mentioned	72 h	[19]
	Adapted centrifuge tubes were used to collect the bone marrow	Not mentioned	24 h	[23]
	Frequent medium change and the diminishing trypsinization time were used to purify the BM-MSCs	Not mentioned	8 h	[21]
Density gradient centrifugation	BM was flushed out from the tibia and femur into lymphocyte separation medium	Not mentioned	72 h	[58]
	BM was loaded on 2 ml lymphodex and centrifuged at 350 ×g for 15 min	Not mentioned	168 h	[20]
Immunomagnetic bead cell sorting	Anti-CD11b-, CD34-, and CD45-conjugated dynabeads were used for immunodepletion	Not mentioned	24 h or 48 h	[16]
	The magnetic beads conjugated to anti-CD11b, anti-CD34, and anti-CD45 antibodies were used for immunodepletion	Not mentioned	48 h	[17]
Fluorescence-activated cell sorting	Cells isolated from BM were labeled with monoclonal antibodies and sorted with FACSAria II	21%, 5%, or 2%	72 h or 96 h	[22]