Figure 2.

Ganoderma triterpenoids (GTs) prevented neointimal hyperplasia after carotid artery ligation. (a) LCAs were excised 14 days after ligation from mice receiving subcutaneous injections of GTs (300 mg/kg/day) or DMSO vehicle control (Con) (n = 5 for each group) immediately after ligation and processed for H&E staining. Unligated RCAs served as the sham control. Yellow dashed lines delineate the internal or external elastic lamellae. A: adventitia; I: intima; L: lumen; M: media. Bar: 50 μm. (b) The intima and media areas were quantified as described in Figure 1. Data are means ± SEM from 6 to 8 serial sections from each artery (n = 5). Statistical significance was calculated using two-way ANOVA and post hoc Tukey's tests, ^∗p < 0.05, ^∗∗p < 0.01.