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. 2018 Apr 15;2018:4973964. doi: 10.1155/2018/4973964

Figure 5.

Figure 5

Effect of DAC on cytokine-producing potential of CD4+ Treg under proinflammatory conditions. Flow cytometric analysis of αCD3/αCD28+ 100U IL-2 + 50 ng/mL IL-1β-stimulated FACS-sorted Treg and Tconv on day 8 of culture. The cytokine-producing potential was analyzed following 4 h stimulation with PMA/ionomycin in the presence of Brefeldin A. Cumulative data on IFNγ-, IL-17a-, and IL-10-producing Treg and Tconv that were cultured in the presence or absence of 1 μM DAC. Mean ± SD are shown. N = 6, Wilcoxon signed-rank test, 2-tailed. ∗ p < 0.05.